alsR [2019-05-24 09:40:52]

transcriptional activator of the alsS-alsD operon

Locus: BSU36020

Isoelectric point: 7.29

Molecular weight: 34.18 kDa

Protein length: 302 aa

Gene length: 909 bp

Function: regulation of acetoin synthesis (alsS-alsD)

Product: transcriptional activator (LysR family)

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Carbon metabolism → Carbon core metabolism → Overflow metabolism
• Information processing → Regulation of gene expression → Transcription factors and their control → Transcription factors/ other

Gene

Coordinates: 3,711,498 3,712,406

Phenotypes of a mutant

• no acetoin production

The protein

Catalyzed reaction/ biological activity

• binds the promoter region of the alsS-alsD operon in the presence of acetate, activates transcription PubMed

Protein family

• LysR family of transcription factors

Effectors of protein activity

• acetate (2 molecules per molecule of AlsR) acts as co-factor that triggers activity PubMed

Structure

• 2H98 (from Acinetobacter baylyi, 34% identity)

Expression and Regulation

Operons

• Genes: alsR-ywrK
  Description: PubMed

Biological materials

Mutant

• AMBs3 (spc), available in Jörg Stülke's lab
• 1A978 ( alsR::cat), PubMed, available at BGSC
• BKE36020 (alsR::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCAATATGCATTCCTTT, downstream forward: _UP4_TGAACAATACTTGCAATCTC
• BKK36020 (alsR::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCAATATGCATTCCTTT, downstream forward: _UP4_TGAACAATACTTGCAATCTC

Labs working on this gene/protein

• Elisabeth Härtig, Braunschweig, Germany Homepage

References

Reviews

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Original publications

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